Assays with only one positive test were repeated. the computer virus Lisinopril (Zestril) only in symptomatic individuals and the total quantity of infections is likely to be considerably higher.5 The first Lisinopril (Zestril) case of COVID-19 in Brazil was reported the 26th February 20204. However, the computer virus was recognized in sewage samples in Santa Catarina State, in the South of Brazil, as early as the 27th November, suggesting the computer virus had been circulating several months earlier, and, probably, among asymptomatic infections.7 This situation is similar to Italy, where the computer virus was found out in sewage samples in Milan, in December 2019, even though the first COVID-19 case was reported two months later, the 21st February 2020.8 We statement here evidence that SARS-CoV-2 may have circulated in the Northeast of Brazil before the first COVID-19 instances were reported in the region. In Aracaju, Sergipe State, in Northeast Brazil, the 1st case of COVID-19 was recorded the 14th March 20204, but we statement here that individuals undergoing routine blood examinations for causes unrelated to COVID-19 experienced SARS-CoV-2 Immunoglobulins (IgM and IgG) before medical instances were reported COCA1 in the State We acquired 987 anonymized serum samples collected from January 2020 to April 2020. Samples had been collected by a private laboratory from individuals undergoing laboratory checks for routine examinations and health inspections for causes unrelated to COVID-19. Most of the checks had been paid by health insurance companies, which usually show the individuals were of top socioeconomic status. All samples were kept frozen in the laboratory blood standard bank at ?80?C and tested for the presence of anti-SARS-CoV-2 IgM and IgG using two in vitro diagnostic checks. The 1st assay was a lateral circulation immunochromatography (Nantong Egens Biotechnology CO, Ltd., China), reported by the manufacturer to have 96.9% sensitivity and 100% specificity. The second assay was a lateral circulation sandwich detection immunofluorescence technology (iChroma2? COVID-19 Antibody), which was used with the Ichroma? II Reader (Bodytech Med Inc., South Korea). The assay is definitely reported to have 95.8% sensitivity and 97.0% specificity.3 All samples were thawed the day of testing using a 37?C thermal bath. Samples were considered positive if they were positive by both assays. Assays with only one positive test were repeated. Only samples with two positive assays were considered positive. The study protocol was authorized by the research ethics committee Federal government University or college of Sergipe and individual consent was waived (CAAE: 36.401.320.0.0000.5546). Fig?1, Table?1,6 Open in a separate window Fig. 1 Analysis of the timeline of events, with the development of SARS-CoV-2 in the world, Brazil and Sergipe Story: Red: our results; Blue: world events; Black: Lisinopril (Zestril) established notification. Table 1 Positive checks participants characteristics (Age, Sex, and area of residence) and of IgM and IgG analysis for SARS-CoV-2, Aracaju/SE, Brazil. thead th valign=”top” rowspan=”1″ colspan=”1″ Sample /th th valign=”top” rowspan=”1″ colspan=”1″ Sample day /th th valign=”top” rowspan=”1″ colspan=”1″ IgM /th th valign=”top” rowspan=”1″ colspan=”1″ IgG /th th valign=”top” rowspan=”1″ colspan=”1″ Sex /th th valign=”top” rowspan=”1″ colspan=”1″ Age /th th valign=”top” rowspan=”1″ colspan=”1″ Area /th th valign=”top” rowspan=”1″ colspan=”1″ Economic range* /th /thead 4916/02/20201.87.7F36AtalaiaA12118/02/20202.43.3F30JardinsA12318/02/20207.914.8F63Treze de JulhoA12418/02/20203.46.1F45FarolandiaB27125/02/20204.40.0M32SuissaB30926/02/20203.90.0F34FarolandiaB30526/02/20203.08.7M73GrageruB30626/02/20201.77.4F29JardinsA46829/02/20204.40.0F41CentroC48329/02/20200.22.5F36FarolandiaB47729/02/20204.40.0F26AtalaiaA47529/02/20201.80.0M48Treze de JulhoA47229/02/20201.70.0F63JabotianaB45429/02/20201.70.0F73Ponto NovoB48601/03/20200.02.4F39CentroC93803/04/20200.12.4F47Coroa do MeioB Open in a separate windows Legends: * A- Neighborhoods with occupants with wages above $ 2000; B- Neighborhoods with occupants with wages $ 300 to $ 1900; C- neighborhoods with occupants with wages below $ 300. The mean (SD) age of the 987 participants was 38.9 (22.2) years, ranging from birth to 90 years and 683 (69.2%) were ladies (Table). Sixteen (1.6%) samples tested positive for both assays, 968 (98.1%) were negative and three (0.3%) were indeterminate. Thirteen of the participants with positive samples were ladies and three males. Seven (43.8%) samples were IgM positive, three (18.8%) IgG positive and six (37.5%) were positive for both (Table). These sero-positive individuals had similar age, gender and residence than individuals reported within the 1st 30 days after the 1st COVID-19 case in the State3. These included 46 RT-PCR positive instances, of which 17 were males (37%) and 29 ladies (63%); of which 34 (74%) were adults aged between 20 and 60 years and 37 (80.4%) lived within in Aracaju City. Aracaju offers reported the highest quantity of COVID-19 instances in Sergipe5. Initial COVID-19 instances occurred in the Southern districts.